Delisa, Matthew

Associate Professor

research

research and scholarship focus

  • Integrates engineering design principles with protein biochemistry, microbiology and modern biotechnology to create microorganisms with new or improved protein machinery for solving problems in human health that cannot be solved using natural systems
  • Protein engineering to engineer new activities or non-natural characteristics into protein frameworks that comprise cellular machinery
  • Folding and post-translational modification of complex, next generation immunotherapeutic proteins from the Immunoglobulin Supergene Family
  • Understand how the structure and function of cellular machines affects the behavior of microorganisms in order to provide a basis from which a protein machine’s functionality can be enhanced or even reprogrammed
  • Functional analysis of existing protein machinery
  • Design and engineering of entirely new protein machinery

research areas

affiliations

faculty appointment in

member of graduate field

other Cornell affiliations

background

educational background

  • Ph.D., University of Maryland, College Park (2000)
  • M.S., University of Maryland, College Park (1998)
  • B.S., University of Connecticut (1996)

professional background

Postdoctoral Fellow, University of Texas, Austin (2001-2003)

awards and distinctions

    • Participant, Transatlantic Frontiers of Chemistry Conference (2008)
    • Office of Naval Research Young Investigator (2006)
    • Beckman Foundation Young Investigator Award (2005)
    • NSF CAREER Award (2005)
    • Technology Review's TR35, Top 35 Innovators Under Age 35 (2005)
    • NYSTAR James D. Watson Young Investigator Award (2004)

publications

selected publications (listing in progress)

  • Fisher A.C. and DeLisa M.P. (2008) Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control. PLoS ONE [PubMed].
  • Fisher A.C., Kim J-Y, Perez-Rodriguez R., Tullman-Ercek D., Fish W., Henderson L.A. and DeLisa M.P. (2008) Exploration of twin-arginine translocation for the expression and purification of correctly folded proteins in Escherichia coli. Microbial Biotechnol (in press).
  • Conrado R., Varner J.D. and DeLisa M.P. (2008) Engineering the compartmentalization of metabolic enzymes: mimicking nature's synergy. Curr Opin Biotechnol (invited submission).
  • Fisher A.C. and DeLisa M.P. (2008) Selection of stability-enhanced protein sequences using the twin-arginine translocation system. Methods Mol Biol (invited submission).
  • DeLisa, M.P. and Haugh, J. (2008) First international conference on biomolecular engineering. Biotechnol Prog 24: 1 (Editorial in special issue covering First ICBE Meeting) [PubMed]
  • Kim, J.-Y., Doody, A., Chen, D.J., Cremona, G., Putnam, D.A. and DeLisa, M.P. (2008) Engineered bacterial outer membrane vesicles with enhanced functionality. J Mol Biol [PubMed]
  • Contreras Martinez, L., Borrero, E.E., Escobedo, F. and DeLisa, M.P. (2008) In silico protein fragmentation reveals the importance of critical nuclei in domain reassembly. Biophys J 94: 1575-88. [PubMed]
  • Mansell, T.J., Fisher, A.C. and DeLisa, M.P. (2008) Engineering the protein folding landscape in Gram-negative bacteria. Curr Protein Pept Sci 9 : 138-149 [PubMed]
  • Contreras Martinez, L. and DeLisa, M.P. (2007) Intracellular ribosome display via SecM translation arrest as a selection for antibodies with enhanced cytosolic stability. J Mol Biol 372: 513-24 [PubMed]
  • Conrado, R.J., Mansell, T.J., Varner, J.D. and DeLisa, M.P. (2007) Stochastic reaction-diffusion simulation of enzyme compartmentalization reveals improved catalytic efficiency for an engineered bacterial propanediol pathway. Metab Eng 9:355-63 [PubMed]
  • Liao, Q., Subramanian, G., DeLisa, M.P., Koch, D.L. and Wu, M. (2007) Pair velocity correlations among swimming bacteria are determined by force-quadrupole hydrodynamic interactions. Phys Fluids19:061701
  • Perez-Rodriguez, R., Fisher, A.C., Perlmutter, J.D., Hicks, M., Chanal, A., Santini, C.L., Wu, L.-F., Palmer, T. and , DeLisa, M.P. (2007) An essential role for the DnaK molecular chaperone in stabilizing overexpressed substrate proteins of the bacterial twin-arginine translocation pathway J Mol Biol 367: 715-30.
  • Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T. and Georgiou G. (2007) Export pathway selectivity of Escherichia coli twin-arginine translocation signal peptides. J Biol Chem 282: 8309-16 [PubMed]
  • Wu, M., Roberts, J.W., Kim, S., Koch, D.L. and DeLisa, M.P. (2006) Collective bacterial dynamics revealed using a three-dimensional population-scale defocused particle tracking technique Appl Environ Microbiol 72: 4987-94.

  • Conrado RJ, Mansell TJ and DeLisa MP. "Engineering multifunctional enzyme systems for optimized metabolite transfer between sequential conversion steps." In: Smolke, C.D., editor. Handbook for Metabolic Pathway Engineering. San Diego: CRC Press.
  • Conrado RJ, Mansell TJ and DeLisa MP. "Engineering multifunctional enzyme systems for optimized metabolite transfer between sequential conversion steps." In: Smolke, C.D., editor. Handbook for Metabolic Pathway Engineering. San Diego: CRC Press.
  • Fisher, AC, Kim, W and DeLisa, MP (2006) Genetic selection for protein solubility enabled by the folding quality control feature of the twin-arginine translocation pathway. Protein Sci 15: 449-458.
  • Contreras Maria L, Martinez-Veracoechea F, Pohkarel P, Stroock AD, Escobedo F and DeLisa MP (2006) Protein translocation through a tunnel induces changes in folding kinetics: a lattice model study. Biotechnol Bioeng 94: 105-117. 
  • Diao J., Young L., Kim S., Fogarty E.A., Wu M., Zhou P., Shuler M.L. and DeLisa M.P. (2006) A three-channel microfluidic device for generating static linear gradients and its application to the quantitative analysis of bacterial chemotaxis. Lab Chip 6: 381-388.
  • Bronstein PA, Marrichi MJ, Cartinhour S, Schneider DJ and DeLisa MP (2005) Identification of twin-arginine translocation system in Pseudomonas syringae pv. tomato DC3000 and its contribution to pathogenicty and fitness. J Bacteriol 187: 8450-8461. [Pubmed]
  • Kim J-Y, Fogarty EA, Lu FJ, Zhu H, Wheelock GD, Henderson LA and DeLisa MP (2005) Twin-arginine translocation of active human tissue plasminogen activator in Escherichia coli. Appl Environ Microbiol 71: 8451-8459. [PubMed]
  • DeLisa MP, Lee P Palmer T and Georgiou G (2004) Phage shock protein PspA of Escherichia coli relieves the saturation of protein export via the Tat pathway. J Bacteriol 186: 366-373. [Pubmed] [Selected as a “Highly Recommended” paper by Faculty of 1000]
  • Bronstein PA, Marrichi MJ and DeLisa MP (2004) Dissecting the twin-arginine translocation pathway using genome-wide analysis. Res Microbiol 155: 803-810. [PubMed]
  • Fisher AC and DeLisa MP (2004) A little help from my friends: quality control of presecretory proteins in bacteria. J Bacteriol 186: 7467-7473. [Pubmed]
  • Masip L, Pan J, Haldar S, Penner-Han JE, DeLisa MP, Georgiou G, Bardwell J and Collet J-F (2004) An engineered pathway for the formation of protein disulfide bonds. Science 303: 1185-1189. [Pubmed] [Selected as a “Highly Recommended” paper by Faculty of 1000]
  • DeLisa MP, Tullman D and Georgiou G (2003) Folding quality control in the export of proteins via the bacterial twin-arginine translocation pathway. Proc Natl Acad Sci USA 100: 6115-6120. [Pubmed] [Selected as a “Must Read” paper by Faculty of 1000]
  • DeLisa MP, Samuelson P, Palmer T and Georgiou G (2002) Genetic analysis of the twin-arginine translocator pathway in bacteria. J Biol Chem 277: 29825-29831. [Pubmed]
  • DeLisa MP and Bentely WE (2002) Bacterial autoinduction: looking outside the cell for new metabolic engineering targets. Microb Cell Fact 1: 5. [Pubmed]
  • DeLisa MP, Wu C-F, Wang L, Valdes JJ and Bentely WE (2001) DNA microarray-based identification of genes controlled by AI-2 stimulated quorum sensing in Escherichia coli. J Bacteriol 183: 5239-5247. [Pubmed]
  • DeLisa MP, Valdes JJ and Bentley WE (2001) Quorum signaling via AI-2 communicates the "metabolic burden" associated with heterologous protein production in Escherichia coli. Biotechnol Bioeng 75: 439-450. [Pubmed]
  • DeLisa MP, Valdes JJ and Bentley WE (2001) Mapping stress-induced changes in autoinducer AI-2 production in chemostat-cultivated Escherichia coli. J Bacteriol 183: 2918-2928. [Pubmed]